| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074959 |
| Chromosome: | chromosome 7 |
| Location: | 1217666 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321200 | (1 of 5) PTHR13271//PTHR13271:SF28 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAACCATTCCTCCTGCTCCCTTCGCGCA |
| Internal bar code: | TGGTTTTTTTACCCTATGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 136 |
| LEAP-Seq percent confirming: | 99.2531 |
| LEAP-Seq n confirming: | 1196 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCGATGTACGTTTGTG |
| Suggested primer 2: | TGAAGATGATGTGGCAGAGC |