| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074963 |
| Chromosome: | chromosome_6 |
| Location: | 4887612 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g279100 | DPY30 | Subunit of chromatin modifying protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAAGCGGGCGCGCCCGCGGCCAAGGCAGAT |
| Internal bar code: | CAGCGGGCCGTGGACGGGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 77.6119 |
| LEAP-Seq n confirming: | 6396 |
| LEAP-Seq n nonconfirming: | 1845 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCCTCTACCTGCGACTG |
| Suggested primer 2: | TGGACTTGTGAGCAATAGCG |