| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074963 |
| Chromosome: | chromosome_6 |
| Location: | 4887679 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g279100 | DPY30 | Subunit of chromatin modifying protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCACAGGGTTGTCCGGCCGCTCCTTGCAC |
| Internal bar code: | TATTTGCGTCGGGGTTTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 764 |
| LEAP-Seq percent confirming: | 99.4183 |
| LEAP-Seq n confirming: | 15381 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCCTCTACCTGCGACTG |
| Suggested primer 2: | ATTTGTAAGTCCGTGTCGCC |