| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074982 |
| Chromosome: | chromosome 8 |
| Location: | 260353 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358555 | (1 of 2) PTHR10625//PTHR10625:SF131 - HISTONE DEACETYLASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGGGGGCCCCCTTCCAATGCGTCCGACA |
| Internal bar code: | AGACAGGTGCGCCGCTAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 99.5085 |
| LEAP-Seq n confirming: | 35229 |
| LEAP-Seq n nonconfirming: | 174 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTCCGCAGATGCTATAA |
| Suggested primer 2: | CAAAAAGACCCCTCGTTTCA |