| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074999 |
| Chromosome: | chromosome 17 |
| Location: | 1215640 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705050 | LIC1 | (1 of 3) PF02931//PF02932 - Neurotransmitter-gated ion-channel ligand binding domain (Neur_chan_LBD) // Neurotransmitter-gated ion-channel transmembrane region (Neur_chan_memb); Ligand-gated ion-channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGACGTGAACGTGACAGCGCTTCTGGAG |
| Internal bar code: | GCACGGAGCGATACGGCCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 882 |
| LEAP-Seq percent confirming: | 96.3004 |
| LEAP-Seq n confirming: | 3462 |
| LEAP-Seq n nonconfirming: | 133 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGACGTCTCAATCTGGTGT |
| Suggested primer 2: | AAAACATCCTCACCACCAGC |