| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075046 |
| Chromosome: | chromosome 3 |
| Location: | 1477776 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g151550 | (1 of 1) IPR000104//IPR029060 - Antifreeze protein, type I // PIN domain-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGGCAGCGGCGGCGAGGACATTGTGCG |
| Internal bar code: | ACGCTTACTGCGGTAGGTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 69 |
| LEAP-Seq percent confirming: | 62.9921 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGCAACCTCAGCAGCTAT |
| Suggested primer 2: | GTGCTGTTGTTGCTGCTGAT |