| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075050 |
| Chromosome: | scaffold 34 |
| Location: | 16713 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre34.g759147 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCGACAGTGGACCCGATGCCAACACTG |
| Internal bar code: | TTGCCGGCTGGAGACACATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 69.1358 |
| LEAP-Seq n confirming: | 224 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTACTTCCTGCCTCACC |
| Suggested primer 2: | GTAGTTGTCCCAGGCGATGT |