| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075078 |
| Chromosome: | chromosome 2 |
| Location: | 1556277 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084750 | (1 of 1) PTHR24012:SF497 - TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGGAACTGTGCTGACGATGTACGCTTA |
| Internal bar code: | AATTTGATGGATAGTGAGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 80.9021 |
| LEAP-Seq n confirming: | 2224 |
| LEAP-Seq n nonconfirming: | 525 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGCACACAAGTACCCCT |
| Suggested primer 2: | AGATGGCTATGAGGGTGGTG |