Insertion junction: LMJ.RY0402.075105_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.075105
Chromosome:chromosome 4
Location:379608
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g217914 WDR65,FAP57,BOP2 (1 of 1) IPR001680//IPR011047//IPR015943//IPR017986 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40/YVTN repeat-like-containing domain // WD40-repeat-containing domain; Flagellar Associated Protein 57 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATCCACCTAGTTCGGCTTGGCTTGGTTT
Internal bar code:CGGGTGTGAGGTACCCTACGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:471
LEAP-Seq percent confirming:99.2172
LEAP-Seq n confirming:507
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTCCAACAACTCAACAGC
Suggested primer 2:CGGCTTGTCATACAACATGG