Insertion junction: LMJ.RY0402.075108_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.075108
Chromosome:chromosome 6
Location:3118906
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g275150 CGL69,TGL11 (1 of 1) PTHR21493//PTHR21493:SF84 - CGI-141-RELATED/LIPASE CONTAINING PROTEIN // F21O3.11 PROTEIN; conserved protein with lipase motif 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTTCCACGGGCCAATGGTCGGATGACGC
Internal bar code:GACTGATATGCTGCCGCTAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:766
LEAP-Seq percent confirming:99.556
LEAP-Seq n confirming:4485
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACACGTTCCCTAGTTCGTGG
Suggested primer 2:GTCAGGCAAGGGCAATTTTA