Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.075165 |
Chromosome: | chromosome 12 |
Location: | 6488874 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g538450 | EPT1 | CDP-Ethanolamine:DAG Ethanolamine phosphotransferase; (1 of 1) 2.7.8.1 - Ethanolaminephosphotransferase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGCTGGCGCCGAACCTCATCACGCTAG |
Internal bar code: | CACAGTTTGGTGGTCGGGGCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 73 |
LEAP-Seq percent confirming: | 6.49922 |
LEAP-Seq n confirming: | 207 |
LEAP-Seq n nonconfirming: | 2978 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCAAGGTATTATGGGCTGA |
Suggested primer 2: | CTCTCCCTAGTTCCGCAGTG |