| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075166 |
| Chromosome: | chromosome 6 |
| Location: | 5833445 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g287750 | PYR8 | Dihydroorotase dehydrogenase; (1 of 1) 1.3.1.14 - Dihydroorotate dehydrogenase (NAD(+)) / Orotate reductase (NADH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCACCTGTGTGCGTGCGTTTCCAGTAC |
| Internal bar code: | ACGAGGCCCTCTGTGAGTTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 94.5978 |
| LEAP-Seq n confirming: | 3152 |
| LEAP-Seq n nonconfirming: | 180 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGTGGGTTGTCTTCACT |
| Suggested primer 2: | GACAGATGATGGTGGTGTCG |