Insertion junction: LMJ.RY0402.075204_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.075204
Chromosome:chromosome 12
Location:725063
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g495100 PSR1 (1 of 1) PF00249//PF14379 - Myb-like DNA-binding domain (Myb_DNA-binding) // MYB-CC type transfactor, LHEQLE motif (Myb_CC_LHEQLE); Phosphorus starvation response 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCAGTTAGGACGCCGTACGCCATAGATT
Internal bar code:AATGTAGGAACCGATGCGCCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:921
LEAP-Seq percent confirming:99.4994
LEAP-Seq n confirming:9541
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGTCCCATCACACACTTG
Suggested primer 2:ATGCCCGTTTGCATAAAGTC