| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075221 |
| Chromosome: | chromosome 3 |
| Location: | 3363279 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166700 | FOR1 | (1 of 3) PTHR23213//PTHR23213:SF214 - FORMIN-RELATED // SUBFAMILY NOT NAMED; Formin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGCCACCGCCGCTACTGCCAGCGCTCT |
| Internal bar code: | GACGGATCGCCGTCCCAGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 59 |
| LEAP-Seq percent confirming: | 71.6216 |
| LEAP-Seq n confirming: | 265 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGGTTCTGCACGGTAT |
| Suggested primer 2: | CCCTGCACGTATCAAGGTTT |