Insertion junction: LMJ.RY0402.075221_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.075221
Chromosome:chromosome 3
Location:4669921
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g177550 (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTCCTCCGCCTCCCGCCTTCAGTGAGC
Internal bar code:TGCCTTCTCCCTCGCCCGTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:633
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:158
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AACTAGCAACAACGTGCGTG
Suggested primer 2:CACATCCACACATACGCACA