| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075231 |
| Chromosome: | chromosome 13 |
| Location: | 4831740 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g605368 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTACATAACAGAGTGACACCGAAACACAA |
| Internal bar code: | CCGTGGCTTAATAATGGTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 847 |
| LEAP-Seq percent confirming: | 99.169 |
| LEAP-Seq n confirming: | 1074 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCATATAAAGTTGCCCGC |
| Suggested primer 2: | CAGGAGCTAAAATGCGGAAG |