| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075233 |
| Chromosome: | chromosome 3 |
| Location: | 7205612 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207901 | (1 of 6) 3.6.4.1 - Myosin ATPase / Actomyosin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCCCCGCACCCTGGAACATTCAAGTAG |
| Internal bar code: | GAGCTATCAGTCCACCCGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 98.4127 |
| LEAP-Seq n confirming: | 372 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCTGCGAATTGATGAAA |
| Suggested primer 2: | CTTCGCATCAAGTTTTGGGT |