Insertion junction: LMJ.RY0402.075243_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.075243
Chromosome:chromosome 7
Location:3823558
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g338550 (1 of 1) IPR002048//IPR006585//IPR006652//IPR008979//IPR011043//IPR011050 - EF-hand domain // Fucolectin tachylectin-4 pentraxin-1 // Kelch repeat type 1 // Galactose-binding domain-like // Galactose oxidase/kelch, beta-propeller // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAACAGCACGCCCGTCATGGCCAGCACCT
Internal bar code:CGAGTATGTCTGGGTCGCTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:840
LEAP-Seq percent confirming:89.1553
LEAP-Seq n confirming:3198
LEAP-Seq n nonconfirming:389
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCTACTCGCTTCACAGGG
Suggested primer 2:GTGTTTTTGTGCGGTTCCTT