| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075278 |
| Chromosome: | chromosome 4 |
| Location: | 3626907 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228650 | (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGGAGGTTTTGGATCACTGCTGGTTTG |
| Internal bar code: | CCTCTTGGGACGAGCAAGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 64 |
| LEAP-Seq percent confirming: | 92.7705 |
| LEAP-Seq n confirming: | 10625 |
| LEAP-Seq n nonconfirming: | 828 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTCAACACACACCCCAGA |
| Suggested primer 2: | GGGATTAGTGTCCACACGCT |