| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075298 |
| Chromosome: | chromosome 9 |
| Location: | 4343594 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394991 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTGCTAATGCGAACCGATGGGGGCGTC |
| Internal bar code: | TCGCCCAGCCAATTTGATAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 62 |
| LEAP-Seq percent confirming: | 3.76164 |
| LEAP-Seq n confirming: | 101 |
| LEAP-Seq n nonconfirming: | 2584 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCCTTACGCTTACCAAG |
| Suggested primer 2: | CAAAGACCGGTACTGGCAAT |