Insertion junction: LMJ.RY0402.075303_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.075303
Chromosome:chromosome 3
Location:4104590
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g173050 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCGGCATTTGCGCGCGCGCCCTAGACAA
Internal bar code:TAGTATTGCGTAGCAGGGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:185
LEAP-Seq percent confirming:99.1525
LEAP-Seq n confirming:351
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AACGTTTGCAATCACAGCAG
Suggested primer 2:TACGAAAGGGGACAAACTCG