| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.075308 |
| Chromosome: | chromosome 16 |
| Location: | 4606966 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687750 | GT90F28,GT90-28 | GT90 family protein 28; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCCCAGCCCTTGGCGCGCAGATCCGCC |
| Internal bar code: | TGTGGCGGGGACTCTGCCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 398 |
| LEAP-Seq percent confirming: | 99.899 |
| LEAP-Seq n confirming: | 2966 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATATGCTGCAGAGATCCG |
| Suggested primer 2: | CCAGCTGCTCTGCTCCTACT |