| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.075314 |
| Chromosome: | chromosome 3 |
| Location: | 2801064 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162150 | (1 of 26) IPR016177 - DNA-binding domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTTGGGGAGCTTGCGGGGTCGGGTCGT |
| Internal bar code: | AGTCGAATGATCTCACGTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 99.0225 |
| LEAP-Seq n confirming: | 2026 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTAAAATTTTTGGGCTGG |
| Suggested primer 2: | ACAACAGCAACAGCAACAGG |