| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075334 |
| Chromosome: | chromosome 9 |
| Location: | 1144129 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400100 | (1 of 1) PTHR10634 - AN1-TYPE ZINC FINGER PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAAGCTGCCATGGATCCTGTGGGCCCT |
| Internal bar code: | CCACGCGGACGTTTACTGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 801 |
| LEAP-Seq percent confirming: | 99.3998 |
| LEAP-Seq n confirming: | 2153 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCTTGTCAGGAAGGGGT |
| Suggested primer 2: | CAACGAATCCAATCATGCAC |