| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075339 |
| Chromosome: | chromosome 17 |
| Location: | 5888622 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739950 | (1 of 1) PF01612//PF13086//PF13087 - 3'-5' exonuclease (DNA_pol_A_exo1) // AAA domain (AAA_11) // AAA domain (AAA_12) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTTTTGAATTGCACGTTTTCCTGTAAC |
| Internal bar code: | GTCGGTGTGAGCCAAAATCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 18 |
| LEAP-Seq percent confirming: | 94.8317 |
| LEAP-Seq n confirming: | 4000 |
| LEAP-Seq n nonconfirming: | 218 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTCAAGGCTTTTGGAAG |
| Suggested primer 2: | CCGTACATCTCCCGACTGAT |