| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075348 |
| Chromosome: | chromosome 13 |
| Location: | 4100775 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591501 | PUS2 | (1 of 2) PTHR11142:SF0 - TRNA PSEUDOURIDINE SYNTHASE-LIKE 1; RNA pseudouridine synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGGTTGCTGCTGCTGCTGCGGCTGTGC |
| Internal bar code: | GTGGATGGACTCGGTGGATGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 440 |
| LEAP-Seq percent confirming: | 98.4526 |
| LEAP-Seq n confirming: | 509 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCATAACACACATTGCG |
| Suggested primer 2: | TGGCTCTCTTTGCGGTAAGT |