Insertion junction: LMJ.RY0402.075350_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.075350
Chromosome:chromosome 5
Location:3355964
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g240700 FAP378 Flagellar Associated Protein 378; (1 of 1) PF00530//PF05572 - Scavenger receptor cysteine-rich domain (SRCR) // Pregnancy-associated plasma protein-A (Peptidase_M43) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGATGCTGATGGTCATCATTTAGTAGGCTA
Internal bar code:GGGCGGAATATAGAAAGTTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:614
LEAP-Seq percent confirming:95.2468
LEAP-Seq n confirming:521
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCCGTACAGCCACCAACT
Suggested primer 2:TTGCGCAAGAGTGATTGTTC