| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075354 |
| Chromosome: | chromosome 16 |
| Location: | 1044848 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649400 | RRM15,CPLD16 | Putative RNA methyl transferase; (1 of 1) PTHR11061:SF12 - RNA BINDING PROTEIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTCCAACCCCCAGCCCAGCTCATGGCCC |
| Internal bar code: | TGAGCGTGTCACAGTCGGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 366 |
| LEAP-Seq percent confirming: | 99.0783 |
| LEAP-Seq n confirming: | 430 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTCCTCAGGCCAACAAT |
| Suggested primer 2: | TTTTTACCGATTGCACACCA |