| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075405 |
| Chromosome: | chromosome 3 |
| Location: | 1147721 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149300 | (1 of 1) 2.8.4.5 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase / Methylthio-threonylcarbamoyl-adenosine transferase B | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTATCTTACTACATTAACATGTACCGCAA |
| Internal bar code: | ACTGTAACGACGGGAGAGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 96.2097 |
| LEAP-Seq n confirming: | 1193 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGTTGTCAGGAGACGAT |
| Suggested primer 2: | ACCAGCAGGATGTCGTTACC |