| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075417 |
| Chromosome: | chromosome 12 |
| Location: | 8767498 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g545600 | (1 of 4) IPR001841//IPR018957 - Zinc finger, RING-type // Zinc finger, C3HC4 RING-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTCAGCTGGCGATGAGCTTGCAAGCGGC |
| Internal bar code: | CCGTGGACGCGAGGCGCCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 122 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTTAACCTCGCTATCTC |
| Suggested primer 2: | GAGGTGAAGGTGAGGAGCAG |