| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075419 |
| Chromosome: | chromosome 3 |
| Location: | 5369384 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184450 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAAGGTGCCAGACACAGCAGCACAGTG |
| Internal bar code: | GGAAGTGCCCGCGTAGGAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 99.0161 |
| LEAP-Seq n confirming: | 4126 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGCACAAGCTGCACATC |
| Suggested primer 2: | AGTCCCGGCTAGGGTACTGT |