| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075420 |
| Chromosome: | chromosome 10 |
| Location: | 1193664 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426250 | (1 of 1) K14800 - pre-rRNA-processing protein TSR2 (TSR2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGAGAGGGGCGTGTGGGATTTGCGGATG |
| Internal bar code: | GCGTGGTTCGGGGGTAATTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 64 |
| LEAP-Seq percent confirming: | 99.4662 |
| LEAP-Seq n confirming: | 559 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
| Suggested primer 2: | CTCAGGAGGGTTGGTAGCAG |