Insertion junction: LMJ.RY0402.075430_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.075430
Chromosome:chromosome 7
Location:2954454
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g332901 (1 of 80) IPR003882 - Pistil-specific extensin-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGTGGCGACTCATTCGGCTGCTGAGGC
Internal bar code:ATCGAGTGGTAAGTGGGCTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:30
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGATGGGTTACAGAACGC
Suggested primer 2:AGTACTAGGCAAGGCTGCCA