| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075434 |
| Chromosome: | chromosome 16 |
| Location: | 5947334 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677001 | (1 of 1) PTHR31596:SF1 - T-CELL ACTIVATION INHIBITOR, MITOCHONDRIAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCAGATGTCGCGCGCACACACACACACA |
| Internal bar code: | GACTCTCCCGGAGGCTGGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 86.8757 |
| LEAP-Seq n confirming: | 748 |
| LEAP-Seq n nonconfirming: | 113 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTTACAGGGGGAAGGAC |
| Suggested primer 2: | CGAGGAGTGACCATGTGATG |