| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075439 |
| Chromosome: | chromosome_8 |
| Location: | 3412589 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g374700 | FAP244 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGACCTTGACCAACGCTTGACCGCTCTCCG |
| Internal bar code: | CTGGGACCTTTGTCGCGGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 764 |
| LEAP-Seq percent confirming: | 97.1874 |
| LEAP-Seq n confirming: | 2246 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTTGTCAGCTTCTTGGG |
| Suggested primer 2: | CACACGCGAATACCTTGATG |