| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075457 |
| Chromosome: | chromosome 5 |
| Location: | 2806862 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237283 | (1 of 7) IPR020850 - GTPase effector domain, GED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGAACGGCGGCACGTACTCTGCCTGCC |
| Internal bar code: | GGGACGGACGTCTACGGGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 636 |
| LEAP-Seq percent confirming: | 99.2589 |
| LEAP-Seq n confirming: | 2143 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACAACACTGAAAAGGGC |
| Suggested primer 2: | GCTTAATGGGCATTCCAAAA |