| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075476 |
| Chromosome: | chromosome 16 |
| Location: | 5887410 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677300 | GOX16 | Glyoxal oxidase 16; (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTTTGGCGGCCCCTTATTACGTGTCGC |
| Internal bar code: | GGATGAACGGGTGCTGCTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 517 |
| LEAP-Seq percent confirming: | 99.0349 |
| LEAP-Seq n confirming: | 1334 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGATGCAATCGGTAAAT |
| Suggested primer 2: | ACCTTGACCGGAAGGTTTCT |