Insertion junction: LMJ.RY0402.075479_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.075479
Chromosome:chromosome 2
Location:3111664
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095058 (1 of 274) IPR020683 - Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAGGTTTCCATAGTCCATCGAACGCGAC
Internal bar code:GGTGTGATCAAAATTCAGAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:75
LEAP-Seq percent confirming:81.8182
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGGCGAACAAGGTAACAT
Suggested primer 2:CAGCCTCCCTAGACTGTTGC