| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075496 |
| Chromosome: | chromosome 2 |
| Location: | 4754412 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102500 | RAD51C | DNA recombination protein; (1 of 1) K10870 - RAD51-like protein 2 (RAD51L2, RAD51C) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCACCTGCCGCCCGGCGACGCCGCGTT |
| Internal bar code: | GGGTTTGGGACCCTTGGTATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 99.8072 |
| LEAP-Seq n confirming: | 1553 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCGCATGTGTTGAATGT |
| Suggested primer 2: | GGCTGCACAGTCACTCACAT |