| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075497 |
| Chromosome: | chromosome 8 |
| Location: | 4387406 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381900 | (1 of 2) PTHR22763:SF71 - PROTEIN Y105C5B.11, ISOFORM B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCACGTTTAAATGTGTTTGCATGCGCAC |
| Internal bar code: | GCGTACTCAAAGCGAGCCCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 163 |
| LEAP-Seq percent confirming: | 61.2299 |
| LEAP-Seq n confirming: | 458 |
| LEAP-Seq n nonconfirming: | 290 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACGTAGTCACGCACATC |
| Suggested primer 2: | GCTTCAGCTTCATCTCCTGC |