Insertion junction: LMJ.RY0402.075529_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.075529
Chromosome:chromosome 13
Location:3731129
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCGTTACCGAAAGCTGGCGCCGCAGTGG
Internal bar code:TCCCTTGGCACGCATATAACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:604
LEAP-Seq percent confirming:99.2191
LEAP-Seq n confirming:2541
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCCAAATTGCCAACTAAC
Suggested primer 2:CACTACCGCACAGGGAGTTT