| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075529 |
| Chromosome: | scaffold 18 |
| Location: | 12281 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g748097 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTTGTTTACTATCAAGTATTTCCATAC |
| Internal bar code: | GCGTCCACCTGGCGACATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 574 |
| LEAP-Seq percent confirming: | 99.6945 |
| LEAP-Seq n confirming: | 5222 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAACACAGGTGAAAGGTT |
| Suggested primer 2: | CCTGCGTGCACTACTCAGAA |