| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.075549 |
| Chromosome: | chromosome 13 |
| Location: | 1929053 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576150 | APN1 | (1 of 1) PTHR11707//PTHR11707:SF3 - L-ASPARAGINASE // SUBFAMILY NOT NAMED; L-asparaginase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGGAGTGCACAGCGCGACCCTGACCGCA |
| Internal bar code: | TTCGGTGCGGATATATGTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 99.7138 |
| LEAP-Seq n confirming: | 6271 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACTGATCGTATCCGCTG |
| Suggested primer 2: | AAGTGAGGGCAGGTTAGGGT |