| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075568 |
| Chromosome: | chromosome 9 |
| Location: | 4559013 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396213 | PSBO | Oxygen-evolving enhancer protein 1 of photosystem II | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCAAAGGCCCTGTGCACAGATTAGTCA |
| Internal bar code: | CTCAAAGGCAACTGGGACGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 60.0922 |
| LEAP-Seq n confirming: | 1173 |
| LEAP-Seq n nonconfirming: | 779 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGAAAGTAGGCTGCATGA |
| Suggested primer 2: | TTTGTACGGCCTTTCCTGTC |