| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075576 |
| Chromosome: | chromosome 1 |
| Location: | 3366266 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAATTAAAGTATTCTTGAATGTTTACACA |
| Internal bar code: | GGGCGTGGCGTGATACGTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 733 |
| LEAP-Seq percent confirming: | 99.759 |
| LEAP-Seq n confirming: | 414 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAATTTGCGGTGGCTGAG |
| Suggested primer 2: | AGTGACAACTGGGATGGCTC |