| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075581 |
| Chromosome: | chromosome_2 |
| Location: | 5627018 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g109333 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGCGTAGAACTGCATACTGCTTTGTGGCG |
| Internal bar code: | ACCCGGGGGTTGCGCAGAGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 163 |
| LEAP-Seq percent confirming: | 87.6972 |
| LEAP-Seq n confirming: | 3892 |
| LEAP-Seq n nonconfirming: | 546 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGAGAAAGCAGCAAAGCA |
| Suggested primer 2: | AATGCCCAACACACACTCAA |