| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075583 |
| Chromosome: | chromosome 12 |
| Location: | 1042645 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g491100 | SDR32 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACACGAAGCCTCGGGCCTCCTGCCGGC |
| Internal bar code: | ATTCGCGACGGCACAGGCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 193 |
| LEAP-Seq percent confirming: | 98.4127 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACAACGACAACATCCAC |
| Suggested primer 2: | GTGCCGTACAGACTGGGTTT |