| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.075609 |
| Chromosome: | chromosome 3 |
| Location: | 6315973 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193450 | (1 of 29) PF00612 - IQ calmodulin-binding motif (IQ) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATACAATGACCTCAACCGACGCTTACCA |
| Internal bar code: | ATTTTAACGAGTCTCAGGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 318 |
| LEAP-Seq percent confirming: | 76.7515 |
| LEAP-Seq n confirming: | 3681 |
| LEAP-Seq n nonconfirming: | 1115 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCACACGCACTTGTTC |
| Suggested primer 2: | TGAACAAACTGCAGCCTGAC |