| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075612 |
| Chromosome: | chromosome 11 |
| Location: | 1090250 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467676 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGGAGTGGGATGGGGAGGAGGGGAAGC |
| Internal bar code: | CCTGCGGGCGTGAACGGAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 74.707 |
| LEAP-Seq n confirming: | 1211 |
| LEAP-Seq n nonconfirming: | 410 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGGTGCAAGACCACTA |
| Suggested primer 2: | CATTTCGTCACTTGCAGCAC |