| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.075639 |
| Chromosome: | chromosome 6 |
| Location: | 7259346 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298326 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTCGGGCGGTCCAGGCGGTCTTGCCTT |
| Internal bar code: | CTTAGAGGCCTGGGTCCTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 711 |
| LEAP-Seq percent confirming: | 99.6815 |
| LEAP-Seq n confirming: | 15024 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGTCAGCTCCCCTAGCA |
| Suggested primer 2: | GTCCAGCCTTTCACATCCAT |